¿How to increase peak response in HPLC?
As we know that the high-performance liquid chromatography is a separation technique in analytical chemistry. In HPLC the system suitability…
¿How to determine Loading Capacity?
Loading in chromatography refers to the quantity of material or sample introduced in an analytical column. The highest possible loading…
¿How to Calculate LOD and LOQ?
LOD as per the Signal-to-Noise Ratio:The signal-to-noise ratio is determined by comparing the measured signals with analytes, with which the…
¿How to avoid peak tailing in HPLC chromatography?
The broad peaks split peaks and variable peak heights in HPLC may be imperfectly filled sample in the sample loop…
¿How many types of pumps are there in HPLC?
High-performance liquid chromatography is an important separation technique in analytical chemistry. The ultimate aim of HPLC analysis is to separate,…
¿How does temperature affect paper chromatography?
Paper chromatography is a technique in chemistry that is used to separate a complex mixture of components or solutes with…
¿What is the HPLC method?
HPLC is a technique used to separation, identification, quantitative and for qualitative analysis of each analyte in a sample depends…
¿What is preparative HPLC?
Preparative hplc involves seperating a chemical reaction product from a mixture of products in a state of high purity, sometimes…
¿What is the principal of HPLC chromatography?
HPLC stands for High-Performance Liquid Chromatography. It is an upgraded form of column liquid chromatography. It is a separation technique…
¿Why is silica polar in HPLC columns?
The surface of silica (glass and sand are essentially all silica) is polar because it is covered with silanols (Si-OH).…