🥇Difference between Isocratic and Gradient elution ✔️HPLC

There are two different modes of separation in high-performance liquid chromatography (HPLC) called isocratic and gradient. Both methods are commonly used in reversed-phase chromatography, normal phase chromatography, and ion-exchange chromatography.Isocratic means that your mobile phase is constant over the full analysis time. Using a gradient elution involves that the solvent mixture is changed throughout the analysis and therefore affects the retention of the components.

The main difference between isocratic and gradient HPLC elution system is that during the process of separation in isocratic elution, the same composition is used and the same polarity of the mobile phase is maintained. In gradient elution, it gradually increases or decreases the polarity of the mobile phase throughout the process of separation.

See also!

Isocratic and Gradient HPLC / Difference between

Difference between Spectroscopy and Chromatography

Difference between TLC and Column Chromatography

👩‍🔬 If you want to know other articles similar to Difference between Isocratic and Gradient elution you can visit the HPLC BASICS

HPLC Basics

Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin