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Advantages and disadvantages of ion-pair chromatography

Reversed-phase ion-pairing chromatography is an extremely useful technique in analytical chemistry to separate the charged molecules that would not be retained in regular RP-HPLC. It can be used for both positively and negatively charged types of analytes and is a sophisticated chromatographic technique.Here some advantages and disadvantages of ion-pair chromatography are mentioned.Advantages of ion-pair chromatography:

  • The separation of ion-pairing chromatography includes two additional variables that can be used to manage the selectivity.
  • It can separate both ionized and non-ionized analytes simultaneously.
  • It can improve peak shapes.
  • This significantly reduces separation time compared to ion-exchange chromatography.
  • The preparation of buffers is simple.
  • Has a high reproducibility of results.
  • It has the broadest selection of carbon chain length for better separation and retention.
  • Easy and quicker preparation compared with gas chromatography.
  • It gives very precise and reproducible results.
  • It can separate the compound in short retention time.
  • It has Low limit of quantification (LOQ) and limit of detection (LOD)

Disadvantages of ion-pair chromatography:

  • More operational complexity
  • Interpretation of results is more challenging
  • Column equilibration takes longer time after changing the mobile phase
  • The cost of analysis is a little high since ion pairing is used to separate the compounds.
  • The ion-pair materials are non-reproducibility from batch to batch.
  • The lower activity and stability.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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