Advantages and disadvantages of ion-pair chromatography

Reversed-phase ion-pairing chromatography is an extremely useful technique in analytical chemistry to separate the charged molecules that would not be retained in regular RP-HPLC. It can be used for both positively and negatively charged types of analytes and is a sophisticated chromatographic technique.Here some advantages and disadvantages of ion-pair chromatography are mentioned.Advantages of ion-pair chromatography:

• The separation of ion-pairing chromatography includes two additional variables that can be used to manage the selectivity.

• It can separate both ionized and non-ionized analytes simultaneously.

• It can improve peak shapes.

• This significantly reduces separation time compared to ion-exchange chromatography.

• The preparation of buffers is simple.

• Has a high reproducibility of results.

• It has the broadest selection of carbon chain length for better separation and retention.

• Easy and quicker preparation compared with gas chromatography.

• It gives very precise and reproducible results.

• It can separate the compound in short retention time.

• It has Low limit of quantification (LOQ) and limit of detection (LOD)

Disadvantages of ion-pair chromatography:

• More operational complexity

• Interpretation of results is more challenging

• Column equilibration takes longer time after changing the mobile phase

• The cost of analysis is a little high since ion pairing is used to separate the compounds.

• The ion-pair materials are non-reproducibility from batch to batch.

• The lower activity and stability.

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