hplc column

Factors affecting column efficiency in HPLC

The column or stationary phase selection is the most significant advance in analytical method development. Without a column, which is stable and high performance, the development of a reproducible and rugged method is not possible. The selection of the column is done based on information about the nature and analysis of solutes.

Usually, a lengthy column gives improved separation because of higher theoretical plate numbers. A column with a 5-µm particle size provides good reproducibility, efficiency, and reliability.The column efficiency is reported as the number of theoretical plates. The efficiency of the column in HPLC is dependent on various factors; some factors that affect column efficiency in HPLC are given below.

  • The activity of the adsorbent
  • Column length
  • The dimension of the column
  • The flow rate of the system
  • Packing of the column
  • Particle diameter
  • Particle size distribution
  • Quality of solvents
  • The temperature of the column

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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