what is hplc

Difference between Chromatography and Spectroscopy

Chromatography is one of the significant tools in analytical chemistry. It includes physical separation of substances in a complex mixture of the diverse velocities at which they move through a stationary phase. As different molecules pass through dissimilar rates in the sample by the mobile phase or the medium, they are separated into a series of bands.

Heating a chemical substance or compound in an electric flame or arc is usually involved in the spectroscopic analysis. Heated compounds have particular wavelengths of light that are based on elements. A spectrophotometer is parallel to the spectrograph, apart from that it recognizes the chemicals by passing it to a light beam through a substance without by burning it. The ultraviolet (UV) or Infrared (IR) is the types of spectrophotometers which are analyzed for particular wavelengths.

The key difference between Chromatography and Spectroscopy is that the chromatography is a technique used to separate complex mixtures, depends on their dissimilar distribution amongst the mobile phase and stationary phase by using different methods. Spectroscopy is the study of the interaction amongst the electromagnetic radiation and substance, using which can analyze the molecule structure.


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