A serological pipette is a volumetric piece of glassware used to quantitatively transfer a desired volume of solution from one container to another.
The pipettes are calibrate to a specified temperature (usually 68°F[20°C] or 77°F[25°C]) either to contain (TC) or to deliver (TD) the volume indicated by the engraved/painted markings on the side of the pipette.
The pipettes marked with the TD mark usually have the desired volume with drainage free; whereas in the case of pipettes marked with the TC mark, the last drop should be blown off or washed off with an appropriate solvent.
- ✨What is a serological pipette?
- Serological pipette correct use
- Serological pipette types
- What is the use of the Serological pipette?
- What is the difference between a graduated pipette and the Serological?
✨What is a serological pipette?
Serological pipettes are those that have a resemblance to the graduated pipette that has terminal discharge. In other words, its graduation culminates at the tip of the instrument. It is a gamma ray sterilized instrument and its maximum capacity is from 5 ml up to 25 ml. It is used in the transfer of cell cultures and in any measurement involving an aliquot.
The material of its manufacture is plastic (polystyrene). It has a design that incorporates a blowing hole, like a cigarette through which it is blown to help dispense the retained liquid. It has the presence of a frosted belt that serves as identification.
Located the upper end is thin, colored around the neck. Body fluids are normally transferred for pathogen studies. It has a filter to prevent infectious agents from coming into contact with the atmosphere. Used in the food, cosmetic industry.
They bring marks (TD) which means free drainage and (TC) uses blown or solvent washing. They are classified into different, there are plastic with an open end, 1 ml, 2 ml, 5 ml, 10 ml and are disposable. Bacteriological, used for dairy products, disposable 1.1 ml, 2.2 ml.
Suction, designed to transfer, mix liquids.
Serological pipette correct use
Understanding serological pipettes is very important in their laboratory applications. These are frequently used by laboratory workers, to transfer liquids whose measurements are from 1mL to 50mL.
Serology is a the scientific study of plasma serum or other body fluids that are refers to the diagnostic identification of antibodies. We have gathered some useful techniques and tips to keep in mind when using serological pipettes.
Do not touch the sterile pipette with the hands or place it on the table, as this may contaminate it."Blown" is a term common meaning emptying the pipette to measure the correct volume.You never want the solution to go up to the cotton plug.When using pipettes serological it is important that the user wears protective clothing, such as a dressing gown laboratory, gloves, glasses, etc.
With a variety of serological pipettes to choose from, they can be used for a wide range of experimental assays.
Serological pipette types
The function of the serological pipettes in laboratories, is to transfer measured liquids in milliliters from 1 to 50.
The pipettes are available in plastic or glass, for the purpose of this post we will focus on single-use polystyrene plastic serological pipettes. We will review three types of serological pipettes, each type of pipette is designed for specific applications.
End pipettes open
The pipettes of open end are specially designed pipettes that have an open end open for use with high viscosity liquids. The open design allows the pipettes suck and dispense viscous liquids quickly and easily.
Therefore, the fast fill and release rates make these pipettes very suitable for use in oils, cosmetics, paints, sludge applications, food and cell cultures.
These pipettes of open end are constructed of polystyrene plastic and provide a safer alternative to glass pipettes.
The liquid vapor and the contamination of fluid to the pipettor and to the sample are reduced significantly with a fiber filter plug located at the end of the pipette tip.
These pipettes do not pyrogenic are sterilized by gamma irradiation and come wrapped individually in thermoformed paper/peelable plastic packaging.
The pipettes are available in 1ml, 2ml, 5ml and 10ml sizes. Color-coded to comply with the ASTM E1380 industrial standard for easy identification, these open-ended pipettes are an excellent choice for efficient liquid handling.
Use mainly in the examination of dairy products, the milk pipettes or bacteriological are designed for use in the field of products dairy and comply with the American Public Health Association standard (A.P.H.A.) for the examination of dairy products.
These pipettes of polystyrene milk are available in sizes of 1.1 mL and 2.2 mL.
These pipettes sterile, non-pyrogenic, single-use bacteriological are sterilized by gamma irradiation and come individually wrapped in paper thermoforming/peelable plastic packaging.
These pipettes use a fiber filter that helps prevent fluid and liquid vapor contamination of the samples and the internal mechanism of the pipette. This pipette is calibrated to deliver (TD) at +/- 2% and complies with ASTM E934 standards.
Pipettes of aspiring
Unlike the open and bacteriological pipettes, aspiration pipettes are completely transparent and have no gradations.
Designed for the liquid transfer and mixing, are used in a wide variety of applications, such as vacuum or pipette aspiration procedures.
These pipettes of single-use polystyrene, non-pyrogenic, non-clogged, are an alternative safer to Pasteur glass pipettes.
These pipettes of all come individually wrapped in a plastic/paper shell thermoformed to protect them against contamination.
Sterilized by gamma irradiation, these pipettes have a sterility assurance level (SAL) of 10-6.
What is the use of the Serological pipette?
Now that we have with the three different types of serological pipettes available, you must have a better understanding of the unique capabilities of each type of pipette.
For major flexibility and convenience, different sizes of pipettes can be used with the same pipette controller for a variety of experimental assays.
With an adequate use of the techniques of handling serological pipettes, they end up being a fundamental tool for the laboratory worker who requires transferring volumes of solutions measured in milliliters in his workplace.
What is the difference between a graduated pipette and the Serological?
This is an pipette possessing its marked volume along the tube. They are necessary in case of requiring accuracy at the time of carrying out the transfer of a volume of liquid from one container to another.
They are located made of plastic or glass and its tip has a conical shape. It also has graduation marks on your body that allows you to visualize the amount of volume from the tip to the point that is observed.
One of the advantages the advantage of small pipettes is that they allow a measurement of fluids with greater accuracy. The big ones, on the other hand, are useful when precision is not really necessary.
Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 milliliters (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz).
Pipettes can be sterile, plastic and disposable or sterilizable, glass and reusable.
The two types of pipettes require another to assist them in the aspiration and dispensing of fluid. Different sizes of pipettes can be used with the same aid of pipette for a variety of experimental assays.
For example, in the in the case of serological pipettes, these can be used to make mixtures of chemical solutions or cell suspensions. As has already been said, it is also they can transfer liquids from one container to another or to stratify the reagents having different densities.
With a careful attention to the level of liquid being aspirated and dispensed, the pipettes serological techniques can be useful tools for transferring exact volumes of milliliter solutions in the laboratory.
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