Factors affecting column efficiency in column chromatography
Column chromatography is the basic and oldest separation technique for separating compounds according to their polarity.
Here are mentioned some factors affecting column efficiency in column chromatography.
Column packing in resolution is yet another significant factor, appropriate column packing is extremely necessary to achieve better results.
The particle size of the adsorbent:
This is also significant factor in the improved separation, if the size of the particles decreases, the separation is improved.
The dimension of the column:
There are different dimensions column are available for the separation, depending on the process, there is a need to select the appropriate column dimensions. Width ratio, height, and length are significant factors, increasing the column length resulting in better separation.
The solvents to be used should be compatible with the columns and the matrix. Usually, a solvent in column chromatography is used with less viscosity, as the viscosity can influence the flow rate of the solvent system and which affects the separation of the component.
Changing the flow rate can also affect the column efficiency and it needs to separate the analytes with an appropriate flow rate.
The temperature of the column:
Temperature is also a vital factor in chromatographic separation because some chromatographic columns are damaged at higher temperatures and proteins can also, be degraded at high temperatures. So maintain the suitable temperature to improve the efficiency of column chromatography.
Equilibration and column washing:
Equilibration and washing of the column can also affect efficiency.
These are the key factors that can affect resolution in column chromatography.
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