what is hplc

Mass spectrometry is the technique in analytical chemistry for measuring molecules and atoms to determine their molecular weight. The information of such weight or mass data is once in a while adequate, much of the time essential, and constantly valuable in deciding the identity of a species. Mass spectrometry (MS) provides information regarding the molecular weight of compound or analyte and, when a combustion analysis, is performed in conjunction with relative percentages of hydrogen, oxygen, and carbon. This is relatively helpful in determining a molecular formula for the compound you are trying to identify.
The advantages of mass spectrometry:

  1. A major advantage of mass spectrometry than other technologies is that it is extremely sensitive.
  2. It is an exceptional technique to identify unknown components in a sample solution.
  3. It can work combining with other techniques, such as high-performance liquid chromatography (LC-MS) and gas chromatography (GC-MS).
  4. It is a very precise, rapid and sensitive method.
  5. It works with very small sample quantities which are in parts per million (PPM).
  6. This gives the relative molecular mass of every molecule.

The disadvantages of mass spectrometry:

  1. The main disadvantage of mass spectrometry is that it is costly, need a skilled technician, and it is not a portable system.
  2. We will unable to differentiate among isomers of the molecule with the same charge-to-mass ratio.
  3. Chiral columns may be required to separate enantiomers.
  4. The disadvantages of the mass spectrophotometer are that it is not nice to recognizing hydrocarbons that generate parallel ions and is unable to separate optical and geometric isomers.

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