Broad peaks:
The presence of unusually broad peaks in high-performance liquid chromatography (HPLC) is a common issue that can affect the accuracy of the separation process. This phenomenon, often referred to as broad peak HPLC, is regularly seen in isocratic separation; however, it can also occur with gradient elution. Factors such as the flow rate of the mobile phase and the particle size of the column's packing material can influence HPLC broad peaks. The smaller the particle, the better the peak resolution. Chromatographers are advised to optimize the composition and flow rate of the mobile phase and to check the column type to mitigate the effect of broad peaks in HPLC.
The occurrence of broad peaks HPLC is due to a variety of factors, which are listed below.
- Rapid changes in the polarity of the mobile phase during gradient elution.
- Presence of precipitate in the sample solution.
- Column overloading due to an excessive sample load.
- Excessive retention time (RT).
- Extra-column volume that is too large.
- Detector response time that is too slow.
- Poor solubility of the sample in the mobile phase.
- Incompletely filled sample loops can contribute to broad peaks HPLC.
- Detector saturation during peak elution.
- Contaminated or deteriorated chromatography column.
- Incorrect pH of the buffer or mobile phase can lead to broad peak HPLC issues.
References
A. Broad Peak Wiki: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography
B. Knauer: https://www.knauer.net/en/search?q=chromatography
C. Kromasil: https://www.kromasil.com/support/faq.php
D. Shimadzu: https://www.shimadzu.com/an/service-support/technical-support/analysis-basics/basic/what_is_hplc.html
E. ChemistryView: https://www.chemistryviews.org/details/education/9464911/What_is_HPLC/
broad peaks
```
👩🔬 If you want to know other articles similar to ¿What are the causes of broad peaks in HPLC? you can visit the FAQ HPLC
You May Be Interested in: