what is hplc

Difference between HPLC and LCMS

Chromatography is a technique of separation in chemistry, where analytes or components are separated through the mobile and stationary phases. HPLC employs liquid chromatography and the combination of the LC and MS has evolved for quantitative analysis of selected components. Compared to HPLC, liquid chromatography-mass Spectroscopy is very accurate, sensitive, and has the potential of liquid chromatography with the mass analysis this is the significant difference between HPLC and LCMC.The joint technique between HPLC and MS is commonly known as LC-MS, Combining both methods decreases error and enhance accuracy, Liquid chromatography-mass spectrometry is valuable in applications that contain a large number of compounds.

Here are some points of difference between HPLC and LCMS.

  • HPLC is a separation technique that employs mainly to separate, identify and determine each component in the sample of a complex mixture.
  • LC-MS is a hybrid system in which a UV or PDA detector of HPLC replaced with a mass spectrometer.
  • HPLC results are less accurate than LCMS.
  • LCMS results are highly accurate than HPLC.
  • HPLC is a time-consuming method than the LCMS.
  • LCMS has a faster analysis than HPLC.
  • HPLC is just a liquid chromatography technique.
  • The combination of a liquid chromatography and mass spectroscopy method is an LCMS.
  • HPLC is less accurate, sensitive than LCMS.
  • LCMS is more accurate, sensitive than HPLC.
  • Using an HPLC the biomolecules, organic molecules, polymers, and ions can be analyzed.
  • Using an HPLC the biomolecules and organic molecules can be analyzed.


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