what is hplc

Difference between Spectroscopy and Chromatography

The key difference between spectroscopy and chromatography is that spectroscopy is the analysis of interaction among analyte and electromagnetic radiation and chromatography is the isolation of mixture into individual compounds with the help of the mobile phase and stationary phase.

Spectroscopy is an interaction of electromagnetic radiation with the component may absorb or emission sample passed from one energy state to another. The photon interacts with the sample while an electromagnetic radiation beam goes through a sample, they can be reflected, absorbed, and refracted. The radiation can affect the chemical bonds and electrons in a sample. In several cases, the absorbed radiation leads to low energy photon emission. Spectroscopy sees how the phenomenon of radiation affects the sample solution.

Absorbed and emitted spectra apply to obtain about the component information since the interaction relies on the wavelength, so there are numerous kinds of spectroscopy, here are some examples of spectroscopy mentioned.Ultraviolet and Visible Spectroscopy, Infrared spectroscopy, Fourier Transform Spectroscopy, Mass Spectrometry, Raman Spectroscopy, Attenuated Total Reflectance Spectroscopy, Laser Spectroscopy, Atomic Absorption Spectroscopy, Electron Spectroscopy, Gamma-ray Spectroscopy, X-ray Spectroscopy.

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