what is hplc

Advantages and Disadvantages of TLC

Thin Layer Chromatography is a technique of separation in analytical chemistry and which used for analysis and identify the unknown analytes/compounds. This is the basic version of HPTLC, in which the sample is applied to a plate of silica and the sample travel with the solvent and separates.Let’s check some advantages and disadvantages of TLC.Advantages of TLC:

  • TLC is a simple and time-saving way to separate the analytes.
  • This is a cost-effective method since it required a minimum sample and solvent.
  • TCL is the method used to separate non-volatile compounds. 
  • Components will separate faster.
  • It can possible to identify the unknown analytes by compared with the standard.
  • Minimum types of equipment are used compared to other techniques.

Disadvantages of TLC:

  • The temperature and humidity can affect the results of TLC, as it is an open system.
  • This only separates the soluble analytes.
  • The result is not reproducible compared to HPLC.
  • The separation length of the stationary phase is inadequate compared to other chromatographic techniques.
  • Thin Layer Chromatography is a manual process, not automated.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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