Loading in chromatography refers to the quantity of material or sample introduced in an analytical column. The highest possible loading for baseline separation varies for each run and relies on the specific retention factor and separation factor, so it is not possible to determine the exact number based on how high the sample number.
To determine load capacity, load the largest volume of sample at a rapid rate until the analyte of interest no longer has the purity we require. Calculate the loading capacity by dividing the mass of the sample component (gm) by the mass of the media bed in the analytical column. After determining the loading capacity (mg or gm) slash by 10% and do again the test.
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