high performance liquid chromatography, broad peaks

¿How to determine Loading Capacity?

Loading in chromatography refers to the quantity of material or sample introduced in an analytical column. The highest possible loading for baseline separation varies for each run and relies on the specific retention factor and separation factor, so it is not possible to determine the exact number based on how high the sample number.

To determine load capacity, load the largest volume of sample at a rapid rate until the analyte of interest no longer has the purity we require. Calculate the loading capacity by dividing the mass of the sample component (gm) by the mass of the media bed in the analytical column. After determining the loading capacity (mg or gm) slash by 10% and do again the test.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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