Difference between paper and column chromatography

Chromatography is a significant biophysical method that allows the separation, purification, and detection of the analytes of complex mixtures for the quantitative and qualitative analysis. Paper chromatography and column chromatography both are used in the separation of components in chromatography.The key difference between paper chromatography and column chromatography is that the column chromatography in which the different solutes of a sample solution are permitted to move with the gravitational force to the absorptive column (The column is usually made of glass or plastic tube, which set vertically and filled with a stationary phase) and each component is being absorbed by the stationary phase.

Paper chromatography that uses a sheet of filter paper or blotting paper as a stationary phase, the sample can introduce as thin-layer chromatography (TLC) and the mobile phase can move up to separate the sample according to its polarity.

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