what is hplc

Advantages of isocratic elution

High-performance liquid chromatography is a separation technique in which isocratic and gradient mode is used to separate the analytes. Isocratic and gradient elution both are a useful model of separation but the isocratic elution is widely used one. In which a single solvent/mobile phase of constant composition is used to separate the compounds.Isocratic elution is usually helpful in the separation of molecules that differ greatly in their affinity for the stationary phase. As long as possible, use isocratic elution, go to gradient only when isocratic doesn’t work.

The advantages of isocratic elution in HPLC are given below.

  • It is simple to use
  • Does not required to column re-equilibrate with the solvent/mobile phase between consecutive injections
  • It has a lower cost
  • This is the most reliable method
  • The isocratic method is transferable
  • No particular HPLC pump necessary

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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