high performance liquid chromatography, broad peaks

Introduction to Thin Layer Chromatography

Chromatography is intended to use to separate the analytes from the mixture of substances. Each type of chromatography works on the similar principle, and each all has mobile phase and stationary phase, Passes the mobile phase throughout the stationary phase and travel the sample components mixture with it.The Thin layer chromatography (TLC) is a popular and broadly used technique of separation due to the cost efficiency, simple to use, rapidity of isolation, ability to analyze many samples concurrently as well as the high sensitivity.

TLC is used for separation, quantification of components from the mixture, identification and the isolation of analytes and it can also be used to separate a particular component on a preparative scale, it has been used in chemistry, toxicology, biochemistry, food science, environmental science, and in pharmacology discipline, a large variety of TLC equipment is available commercially in the market.


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