high performance liquid chromatography, broad peaks

¿How to Calculate LOD and LOQ?

LOD as per the Signal-to-Noise Ratio:
The signal-to-noise ratio is determined by comparing the measured signals with analytes, with which the sample has low concentrations with blank specimens and the analyte can be detected reliably by establishing a minimum concentration.


LOD as per the visual evaluation:
The identification limit is calculated by analysis of analyte with recognized sample concentration and by setting up the lowest level on which the sample can be consistently detected.
LOD as per the standard deviation of response and the slope:
The LOD can be expressed as - LOD=3 X SD/Slope.
LOQ (limit of quantification)


LOQ as per the Signal-to-Noise Ratio:
The signal-to-noise ratio is determined by comparing the measured signals from the analytes, with which the sample has low concentrations with the blank sample and by setting up at least concentration on which the sample can be constantly quantified. 10:1 a normal signal-to-noise ratio.


LOQ as per the visual evaluation:
LOQ is analyzing by analysis of analyte with recognized concentration and by setting the least level of the analyte to quantify with suitable precision and accuracy.
LOQ as per the standard deviation of response and the slope:
The LOQ can be expressed as - LOQ=3 X SD/Slope.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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