Column chromatography is a general technique that is used to separate the compounds from the complex mixtures. It can use small or large-scale column chromatography to separate and purify the analytes. Column chromatography has two phases, a mobile phase (liquid) and a stationary phase (solid). The sample mixture of compounds travels with the mobile phase through the stationary phase and basis of different degrees of adhesion it separates. Advantages of column chromatography:
- Using column chromatography all kinds of complex mixtures can be separated.
- Any amount of mixture can be separated by column chromatography.
- A broad range of mobile phases.
- Analytes can be separated and reused, in preparative type chromatography.
- It can be possible to run automation.
- This is a robust method.
Disadvantages of column chromatography:
- It takes more time to separate the compounds.
- Higher quantities of solvents are essential, which is more expensive.
- Automation makes more complex and costly.
