what is hplc

Affinity Chromatography - Advantages and Disadvantages

Column chromatography is a general technique that is used to separate the compounds from the complex mixtures. It can use small or large-scale column chromatography to separate and purify the analytes. Column chromatography has two phases, a mobile phase (liquid) and a stationary phase (solid). The sample mixture of compounds travels with the mobile phase through the stationary phase and basis of different degrees of adhesion it separates. Advantages of column chromatography:

  • Using column chromatography all kinds of complex mixtures can be separated.
  • Any amount of mixture can be separated by column chromatography.
  • A broad range of mobile phases.
  • Analytes can be separated and reused, in preparative type chromatography.
  • It can be possible to run automation.
  • This is a robust method.

Disadvantages of column chromatography:

  • It takes more time to separate the compounds.
  • Higher quantities of solvents are essential, which is more expensive.
  • Automation makes more complex and costly.


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