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Affinity Chromatography - Advantages and Disadvantages

Column chromatography is a general technique that is used to separate the compounds from the complex mixtures. It can use small or large-scale column chromatography to separate and purify the analytes. Column chromatography has two phases, a mobile phase (liquid) and a stationary phase (solid). The sample mixture of compounds travels with the mobile phase through the stationary phase and basis of different degrees of adhesion it separates. Advantages of column chromatography:

  • Using column chromatography all kinds of complex mixtures can be separated.
  • Any amount of mixture can be separated by column chromatography.
  • A broad range of mobile phases.
  • Analytes can be separated and reused, in preparative type chromatography.
  • It can be possible to run automation.
  • This is a robust method.

Disadvantages of column chromatography:

  • It takes more time to separate the compounds.
  • Higher quantities of solvents are essential, which is more expensive.
  • Automation makes more complex and costly.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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