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Advantages and Disadvantages of Gradient Elution in HPLC

The elution strength increases throughout separation by gradually changing the mobile phase composition in gradient elution. Thus, the time of analysis is decreases and the quality of separation and detection limit improves. Even if the gradient method is more complex compared with isocratic elution.Advantages of Gradient Elution:

  • Gradient HPLC mode has ability to separate complex samples.
  • This method gives increased sensitivity.
  • Gradient elution has shorter analysis times and gives much narrower chromatographic peaks.
  • It gives an improved resolution.
  • It can reduce the column degradation because of strongly retained analytes.
  • This is the best method compared to an isocratic elution to clean the column.

Disadvantages of Gradient Elution:

  • To flow the mobile phase in gradual elution, a special HPLC pump is required, which is more expensive.
  • Between consecutive injections, it needs to be re-equilibration with the initial mobile phase composition.
  • There is a method transfer problem since instruments vary in their dwell volume.
  • Precipitation is possible when using multiple proportional valves.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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