what is hplc

Advantages of TLC over Paper Chromatography

TLC is a chromatographic method of separation of the analytes or components in the mixture of samples. TLC consists of two phases, one mobile and one solid phase, in which the TCL plate acts as a stationary phase.
Paper chromatography is the types of chromatography, which are used qualitatively for the separation and identification of sample components, or analytes, that can be colored or especially pigments. It runs on a piece of paper that acts as a stationary phase on which the separation occurs. Thin-layer chromatography is parallel to paper chromatography, TLC and paper chromatography as compare with each other, The TLC has a wide range of adsorbent, depending on sample and development. After development sample can be easily separated from TLC. Thin-layer chromatography offers many advantages over paper chromatography, some of which have been mentioned here. 

  • Time-saving method.
  • The sensitivity of detection is more in TLC.
  • Rigid support.
  • The option of support.
  • Simple Setup.
  • The amount of the sample application is in minute quantity (in microlitre).
  • Strong reagents can be used for the purpose of identification.
  • Choice of spray reagents.
  • Higher sample throughput and lower analysis time.
  • If required for spot development, the thin-layer chromatography plates can be heated.

In short, the thin layer, chromatography provides several advantages for separation than the paper chromatography. 


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