Waters HPLC

Introduction to Waters HPLC

Waters HPLC Company is a publicly traded Analytical Research laboratory tool and also software program business headquartered in Milford, Massachusetts. The business uses more than 7,400 individuals, with producing centers situated in Milford, Taunton, Massachusetts; Wexford, Ireland and Wilmslow, Cheshire. Waters has Sites in 35 nations worldwide consisting of Frankfurt, Singapore, India, Germany as well as in Japan.

Waters markets to the laboratory-dependent company in these market areas: liquid chromatography, mass spectrometry, supercritical fluid chromatography, lab informatics, rheometry and also microcalorimetry.

History of Waters HPLC

The business was started by James (Jim) Logan Waters as Waters Associates in a workplace in the basement of a police headquarters in Framingham, Massachusetts, in 1958.

Waters signed up in the V-12 Navy University Training Program, a police officer training program, and finished from Columbia College as an ensign with a B.S. level in Physics in 1946. After stints as a college mathematics educator, Naval police officer, task engineer, and also business owner, Waters created Waters Associates in 1958. The fledgling company's initial offices were in the leased basement of the Framingham, Mass. police headquarters. Throughout these years, Waters Associates was what is now described as a research shop. Business would certainly acquire Waters and also his five employees to construct one-of-a-kind tools for various objectives. Very early products included a boiler feedwater fire photometer, a balloon hydrometer, a nerve gas detector, a lab refractometer and also procedure control refractometers. While from its beginning the company had actually been self-financed, with profits from an earlier service sale, Waters opened Waters Associates to exterior ownership in 1962.

The company's initial major break came when Dow Chemical got among Waters' first gel permeation chromatography instruments, Dow Chemical made an added investment of $400,000. By 1979, Dow Chemical had achieved virtually 25 percent ownership in Waters.

In 1967, the ALC 100, the first Waters HPLC system, was given market. According to Leslie S. Ettre in a review about Jim Waters, the LC system was formally introduced at the 1968 Pittsburgh Meeting. It was a benchtop system furnished with a Milton Roy pump, syringe shot, and also 2 detectors: a Waters differential refractometer as well as a UV detector from the Lab Data Control (LDC) Co

In 1969, Dimitri D'Arbeloff, then head of state of Millipore Corporation, joined the corporation's board of directors. Millipore's equity capital subsidiary made a $600,000 equity investment in Waters.

Waters next luck can be found in 1972 when Dr. Helmut Hamberger, primary post-doc for Nobel Laureate Dr. Robert Woodward of Harvard University, looked for Jim Waters' aid to the first synthesis of vitamin B12. Dr. Hamberger wanted to purify the positional isomers, which were required to offer him the best substance for the lasts of the synthesis. Dealing With Dr. Hamberger, the pair took 2 days to create a separation, 5 even more days to acquire larger columns to scale up the separation, and 3 more days to prep his product. Ultimately, the two had isolated and cleansed 200 mg of the forerunner substance.

In 1972, Waters Associates appointed Frank Zenie president. A year later, head office moved from Framingham to a semi-rural 26-acre (110,000 m2) website in Milford, Massachusetts. Waters became chairman, and proceeded because duty till the company merged with Millipore in 1980, and also was rechristened the Waters Chromatograph Department. The sought-for synergies between the two business never ever emerged, nevertheless. And also, in 1993, Waters returned to freedom under the management of Chairman, Head Of State, as well as President Douglas A. Berthiaume.

1. In 1997 Waters entered mass spectrometry market with purchase of Micromass for $176 million.
2. In 2006, Waters got Vicam, supplier of bioseparation and also rapid discovery products for enhancing food safety as well as high quality.
3. On 11 June 2012, Waters India commemorated its silver jubilee anniversary in India.
4. In January 2020, Waters obtained Andrew Alliance, a pioneer firm in specialty research laboratory automation modern technology, consisting of software and also robotics.
5. In September 2020, Waters introduced Udit Batra, Ph.D. as the business's Head of state as well as Ceo.

Waters HPLC Parts

Waters’ main product brands include: ACQUITY UPLC systems, ACQUITY UPC² Systems, Xevo mass spectrometry systems, Synapt MS systems, Synapt HDMS systems, XTerra HPLC columns, XBridge columns, ACQUITY UPLC columns, Alliance Waters HPLC systems, Empower chromatography and MassLynx mass spectrometry software, Oasis sample preparation products, NuGenesis lab management system (LMS).

Waters hplc column

Waters offers a wide array of column choices and formats, including HPLC, UPLC, UHPLC, GPC, SFC, and SEC, all built on reliable quality manufacturing that ensures that methods developed today will have the same repeatable results tomorrow.

• ACQUITY UPLC Columns

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

SEC is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.

The pore size and volume of the SEC particles used in the packed column drives this isocratic based technique, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.

• Atlantis Premier Columns

Next-generation hybrid particle and surface technology for increased polar analyte retention.
Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.

Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.

• Atlantis Columns

Reversed-phase and HILIC HPLC Columns for superior polar compound retention.
Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.

• ACQUITY APC Columns

Achieve superior speed and resolution for polymer characterization.
Achieving superior performance and reliability with high resolution polymer characterization can be a challenge. ACQUITY Advanced Polymer Chromatography (APC) Columns are designed for polymer scientists like you, with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods. With these columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.

The Ethylene Bridged Hybrid (BEH) particles used in ACQUITY APC Columns enable rapid solvent switching and the ability to use multiple conditions for the same bank of columns. Conventional polymeric stationary phases are dedicated to a single set of analytical conditions and solvent because the chromatographic bed is vulnerable to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns allow for maximum robustness and method development flexibility.

• ACQUITY Premier Columns

Stop looking and start seeing – no compromise required.
Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

• Enzymate Pepsin Column

Faster, more efficient online protein digestion.
Fast, efficient, and reproducible online pepsin digestion is critical in experiments that require more peptide coverage. With the Waters Enzymate BEH Pepsin Column, you can easily incorporate online protein digestion into existing HDX- MS workflows.

The Enzymate BEH Pepsin Column efficiently digests intact proteins into peptides in an online HDX system, typically in about 30 seconds. The peptic peptides are then retained on a trapping column and refocused onto a sub-2-μm ACQUITY UPLC Column for elution into high-resolution mass spectrometry.

• GPC Columns

Organic-and aqueous-based size separations.
Gel permeation chromatography (GPC) is a chromatographic technique that separates molecules based on their relative size in solution and is often used by scientists analyzing polymers (plastics or other synthetic polymers) using either organic or aqueous eluents. With GPC analysis, you can determine polymer physical properties by characterizing the polymer’s molecular weight distribution.

For more than 50 years, Waters has been the market leader in GPC analysis, providing the highest quality columns and applications support for both organic and aqueous-based size separations. With Waters comprehensive GPC Column selection, you can be certain that the GPC Column or column bank you choose will be compatible with temperature, solvent, and polymer type.

• Gen-Pak FAX Columns

Highest resolution available for anion-exchange HPLC of oligonucleotides.
Gen-Pak FAX Columns are designed to perform high-resolution analysis and purification of nucleic acid species such as DNA restriction fragments, polymerase chain reaction (PCR) products, plasmids, and synthetic oligonucleotides.

Waters Gen-Pak FAX Column contains a weak anion exchanger based on DEAE functionalized non-porous resin. It contains 2.5 μm particles and is well suited for analytical and micro-preparative applications.

• MassPREP On-Line Desalting Cartridges

Achieve reliable, reproducible results by effectively desalting proteins.
Mass spectrometry is a powerful tool for intact protein analysis. However, salts in your sample can compromise analysis quality by interfering with ionization and/or form adducts with the investigated proteins. You can significantly minimize or remove the introduction of extraneous salt ions into the mass spectrometer with minimal sample handling using MassPREP On-line Desalting Cartridges.

MassPREP On-line Desalting Cartridges are an effective tool for desalting acidic, basic, and large globular proteins. The MassPREP desalting strategy provides an easy, fast, and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high-resolution ESI-MS structural analysis — enabling you to achieve reliable, reproducible results.

• Nano and MicroFlow LC-MS Columns

Generate high-quality peptide and protein separations.
The ability to reproducibly generate high-quality peptide and protein separations is critically important in proteomics research and biopharmaceutical characterization. Waters Ethylene-Bridged Hybrid (BEH), Charged Surface Hybrid (CSH), and High Strength Silica (HSS) peptide and protein nano columns help address these demanding needs. Combining columns with low retentive trap columns results in highly efficient separations with an increased number of peak identifications.

Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems. The selected stationary phases of these analytical columns fully leverage the separation power of sub-2–µm particle technology at flow rates between 100 nL/min and 50 µL/min, withstanding 15,000 psi, and have an inner diameter between 75 µm – 300 µm. This delivers the efficiency and selectivity required for complex separations like the discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.

• Nova-Pak Columns

Scale-up from analytical to prep, simplified.
Reducing solvent consumption while maintaining resolution for complex mixtures is important, but that’s a challenge with larger particle sizes. With the smaller particle sizes and shorter column lengths in Nova-Pak Columns, those problems are a thing of the past. The bonded phases of Nova-Pak Columns are available in 4 µm and 6 µm particle sizes, offering high resolution and faster, more efficient chromatography over columns packed with larger particle sizes.

Nova-Pak Analytical Columns with 4 µm particle size packing offer a range of length options with 75, 100, 150, and 300 mm length steel columns. The semi-preparative Prep Nova-Pak HR Columns are packed with 6 µm particle size packings for unparalleled separation possibilities. The high-efficiency packing of Prep Nova-Pak HR Columns provides faster separations with less solvent for the added advantage of more concentrated fractions—reducing your preparative chromatography cost. To ensure batch-to-batch reproducibility, all Nova-Pak C18, C8, Cyano and Phenyl Columns are packed according to stringent quality control (QC) procedures in Waters cGMP manufacturing facility.

• Preparative HPLC Columns

Increase throughput with preparative productivity and predictability
As a medicinal chemist working on a challenging drug discovery project or purification scientist attempting to maximize yield, your background in chromatographic separations has typically meant working with other chemists to successfully isolate compounds. However, as fewer drugs are approved for market release, your laboratory must refine your drug discovery or purification processes and use more effective methods to increase throughput.

As your lab works to achieve fast, efficient, lab-scale separations and greater throughput, Waters Preparative HPLC Columns can help you attain predictable performance and easily scale from UPLC, UHPLC, or HPLC screening to lab-scale purification. With the proprietary Optimum Bed Density (OBD) design of Waters Preparative HPLC Columns, you can ensure consistency in column-to-column performance and increase productivity through higher recoveries and longer column lifetimes – making lost samples, repeat purification runs, and poor scalability from small to large volume columns a thing of the past.

• Protein-Pak Columns

Time-tested and proven LC columns for bioseparations
Achieve high-quality LC-based analyses and lab scale purifications of a wide range of peptide and protein biomolecules using Waters Protein-Pak Ion Exchange (IEX) and Hydrophobic Interaction Chromatography (HIC) HPLC and UPLC Columns.

Meet your workflow requirements with a variety of chemistries that include strong and weak anion and cation-exchanges, and a hydrophobic interaction offering. Protein-Pak LC Columns contain either well-performing silica or methacrylate-based, ion-exchange, size-exclusion, and hydrophobic interaction based separation columns for analysis or lab-scale purification of targeted biomolecules.

• Resolve Columns

High resolution spherical packings
Non-endcapped Resolve packings are significantly different from Waters other packing materials in that they typically provide higher retention of polar compounds and complement those of Nova-Pak and µBondapak chemistries. Resolve C18 and silica columns are available in 5 µm and 10 µm spherical packings for applications requiring higher resolution than what is achievable using irregularly shaped chromatographic particles. The non-endcapped silica surface offers a balance of retention for separation of polar compounds.

• SFC Columns

Normal-phase alternative selectivity with reversed-phase ease and reliability
Accessing the power of normal-phase chromatography with the ease and reliability of reversed-phase chromatography is critical for separation scientists like you doing Supercritical Fluid Chromatography (SFC) applications.

With the Torus, Trefoil, and Viridis SFC Column chemistries and Waters SFC instrumentation, now your lab can achieve normal-phase alternative selectivity with reversed-phase ease and reliability - all while ensuring ease of scalability, column-to-column consistency, and reduced method development times. With these high-quality achiral and chiral SFC column chemistries, your lab can address difficult achiral and chiral separations, deliver novel selectivity and robustness, and achieve unequaled speed and unparalleled confidence.

• Shodex RSpak Columns

Ideal for polymeric reversed-phase analysis
Shodex RSpak Reversed-Phase HPLC Columns are packed with porous polymeric material that are stable at a pH range of 2–12 and deliver an alternate selectivity to silica-based columns. The wide pH range makes the chromatographic analysis of basic compounds much easier because it is possible to analyze samples at a higher pH without the need for ion-pairing agents in the elution solvents.

These columns are packed with a more hydrophilic polymethacrylate stationary phase (DS-613) and work well with mobile phases containing relatively high concentrations of water. The DS-613 is a minimally hydrophobic sorbent column packing. Similar to conventional polymer-based materials, the DS-613 sorbent works well with samples that are more hydrophobic than hydrophilic, and as such, require relatively high concentrations of organic modifiers be present in elution solvents.

• SunFire Columns

Superior mass loading at low pH
High efficiency at a low pH can be challenging. Waters SunFire HPLC Columns, available in analytical and preparative dimensions, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.

SunFire Analytical Columns are available in 2.5 µm to 10 µm particle sizes across two reversed-phase column chemistries, C18 and C8, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in 5 and 10 µm particle sizes for preparative normal-phase purification.

• µPorasil/Porasil Columns

Normal-phase separations for a wide array of sample types
μPorasil and Porasil particles were one of the first commercially available, fully porous packing materials used for LC separations. In contrast to the reversed-phase separation ability of μBondapak C18, the non-bonded, silica-based material in μPorasil Columns was produced to provide normal-phase separations for a wide array of sample types.

With a range of different particle size offerings, you have the option of scalability for preparative application with µPorasil column as a QC tool.

Waters HPLC models

Data is critical in scientific research as evidence-based inquiries intensify. Responses call for precise dimensions for exact results to acquire the information to make informed choices.

Scientists across numerous markets rely upon Waters LC and also LC-MS systems to identify and also comprehend chemical parts that help answer their important questions: Is the item safe? Can the condition be comprehended? Can I fulfill my governing requirements?

With Seas as your dimension partner, you're sure to get the customized remedy you need to attain your operations as well as analysis demands. Figure out even more regarding our Stability, Adaptability, Performance, Specialized, as well as Purification LC solutions that are purpose-built for your top priorities.

Dependability	Flexibility	Performance	Specialty	Purification
Accurate analysis for all.Optimized for 3-5-µm column chromatography	Adaptable to your sample complexity. Optimized for 2.5-5-µm column chromatography	The very best in chromatography. Optimized for sub-2-µm column chromatography	Specialized solutions for your unique needs.	A wide range of scale and throughput capabilities.

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Waters hplc detectors

Waters enables you to assess a wide range of substances by offering a huge portfolio of modern technologies. These detectors have a variety of choices, consisting of circulation cells as well as nebulizers, to match your separation scale and aid you to attain the objectives of your analysis. Our technologies are identified with level of sensitivity, stability and functionality, providing you all you require to tackle your laboratory's LC difficulties.

ACQUITY QDa Mass Detector ACQUITY QDa Detector is a mass detector built around the needs of analytical scientists for chromatographic analysis. Robust, reliable and requiring no sample adjustments, it integrates with your current LC, ACQUITY UPLC, UPC2 and purification systems.

2998 Photodiode Array (PDA) Detector Add spectral conformation to your absorbance detection methods while still maintaining high sensitivity and a wide linear dynamic range.

2489 UV/Visible (UV/Vis) DetectorA versatile, dual-wavelength absorbance detector that offers simplicity for routine UV-based applications and high sensitivity for low-level impurity analysis.

2475 Fluorescence (FLR) Detector Delivers unsurpassed sensitivity and selectivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag.

2414 Refractive Index (RI) Detector Offers high sensitivity, stability, and reproducibility, making this detector the ideal solution for the isocratic analysis of components with limited or no UV absorption.

2424 Evaporative Light Scattering (ELS) Detector A complementary detection technique for semi- and non-volatile compounds with little or no UV absorbance. Compatible with both isocratic and gradient methods.

3465 Electrochemical Detector for LC Systems Provides sensitivity for a variety of applications needs. It is configurable with a variety of flow cells for variable volumes, reference electrodes and working electrodes.

2432 Conductivity Detector for HPLC Systems Features a multi-electrode flow cell that offers exceptional sensitivity and stability for single-column or suppressor-based ion chromatography.

Waters Preparative HPLC

Waters Prep HPLC

HPLC Waters