what is hplc

Difference between TLC and HPTLC

TLC and HPTLC are separation techniques of chromatography used to separate the non-volatile analytes or components in the sample mixture. HPTLC process phase is similar to the traditional TLC separation technique. The characteristic of the separation plate is the primary distinction between the TLC and HPTLC. The HPTLC plates depend on enhanced silica with a considerably smaller size particle used for traditional thin layer chromatography. The smaller particle size of silica in HPTLC increases the sensitivity of detection and speed of analysis, and it also permits a smooth surface and a high packing density.
HPTLC is a high-tech version of traditional TLC. Generally, after spotting of samples on the plate for the separation the TLC plates are immersed in a jar. HPTLC usually mechanize this process which permits very accurate sample applications and detection.Here are some points of difference between TLC and HPTLC.

  • The full form of TLC is thin layer chromatography.
  • This is a time-consuming method.
  • It has manual spotting of the sample lead to error.
  • This is a basic technique of separation of non-volatile analyte or components in the complex mixture.
  • While the sample has equivalent properties, the TLC method gives a bad resolution.
  • The TLC plate can be prepared by the glass, plastic, or paper it's applied with an absorbent material which contains large particles.


  • The full form of HPTLC is high-performance thin layer chromatography.
  • This is a time-saving method.
  • It is Autosampler, the accuracy is high.
  • This is an advanced form of thin layer chromatography that can automate the analysis of the sample.
  • HPTLC method gives high resolution as compared to TLC.
  • There are very small particles of adsorbent material used in HPTLC plates.


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