what is hplc

Difference between TLC and Column Chromatography

In chromatography, separating the complex mixture of different compounds into individual compounds is a significant tool. Sometimes a reaction can produce a degradants product other than the desired product, or after analyzing reaction products, a component of the mixture can require being separated after the reaction is completed. Of all the methods to separate, thin layer chromatography and column chromatography is some of the most useful. Thin-layer chromatography is normally utilized for separating the number of mixes in a sample just as their relative polarities rather than physically separating them, which can be practiced with column chromatography. Both methods are utilized to separate the analytes that are generally non-volatile.

Here are some points of difference between TLC and Column Chromatography.

  • TLC has a stationary phase of alumina or silica gel.
  • Column chromatography is packed uses its stationary phase with an appropriate matrix material, such as silica.
  • TLC is carried out against gravity.
  • Column chromatography is run under gravity.
  • TLC uses for analytical purposes.
  • Column chromatography uses for the preparative purpose.
  • TLC required less time to separate than the column chromatography.
  • Column chromatography takes more time to separate than the TLC.
  • TLC needs less quantity of solvent to separate the analytes.
  • Column chromatography required more amount of solvent.
  • TLC needs a more polar solvent compared to the column chromatography.
  • Column chromatography needs less polar solvent compared to the TLC.


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