what is hplc

Difference between TLC and Paper Chromatography

TLC and paper chromatography both are separation techniques of chromatography, which are used to separate the components or biomolecules such as carbohydrates, amino acids, and proteins. As a stationary phase, the paper chromatography uses a cellulose paper, and the separation mechanism depends on the solid-liquid adsorption.The same adsorption mechanism uses in thin layer chromatography, depending on solubility in the mobile phase the molecules or components are separated on the stationary phase. TLC uses the stationary phase as alumina, silica, and its compositions, whereas in paper chromatography, as a stationary phase paper is being used.

Thin Layer Chromatography:TLC is another separation technique of chromatography, which works based on the solid-liquid adsorption of compounds. It has a stationary phase of silica gel or alumina and a solvent in the form of a mobile phase. TLC and paper chromatography use both to determine the sample retention factor.

Paper Chromatography:Paper chromatography is a separation technique of chromatography that uses to separate the different compounds depends on the compound's solubility and liquid-liquid adsorption. It uses a paper as its stationary phase.


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