what is hplc

Difference between HPLC and HPTLC

Both HPLC and HPTLC are the separation techniques of chromatography operating on the same principles of separation.

HPLC: HPLC is known as high-pressure liquid chromatography and also known as High-performance liquid chromatography, it is an analytical technique in chemistry that is used to separate, determine, and identify each component in the complex mixture. It depends on a pressurized mobile phase provided by the pumps in which the sample mixture is included and it passes through the HPLC column. Every analyte in the complex mixture interacts with the absorbing material in a different way, which has dissimilar flow rates for the different analyte and leads to the separation of the analyte or components.

HPTLC: High-performance thin-layer chromatography is the advanced form of TLC and contains the use of chromatographic layers of cutting-edge separation efficiency for all stages of the process and the use of state-of-the-art instrumentation, reproducible chromatogram, accurate sample application, and software-based evaluation. HPTLC is a complete concept in which a broad, standardized method depends on scientific facts and for the quantitative and qualitative analysis uses of validated methods.The main difference between HPLC and HPTLC is that HPLC permits quantitative separation of analytes in a sample of a complex mixture, whereas HPTLC does not allow.Here are mentioned some differences between the HPLC and HPTLC.

High-performance liquid chromatography (HPLC):

  • HPLC is known as high- performance liquid chromatography.
  • It permits quantitative separation of analytes in a sample.
  • In the HPLC system, for separation, a column is used which is filled with the stationary phase and mobile phase passes through it.
  • Analytes can be separated as per their interaction with the column and based on differences in their polarity.

High-performance thin layer chromatography (HPTLC):

  • HPTLC is known as High-performance thin-layer chromatography.
  • It does not permit quantitative separation of analytes in a sample.
  • The complex mixture sample spots on the plate of TLC and is located in a chamber which has mobile phase, after that the sample is permitted to move the plate upwards with the mobile phase.
  • Components can be traveled as per their polarity.


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