Hplc Column Comparison Guide
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Hplc Column Comparison Guide

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There are so many different C18 columns to choose from, that finding the right column for a particular separation can be very time consuming and expensive. Two apparently similar C18 phases can give very different results.

Hplc Column Comparison Guide

For example, Figure 1 compares the separation of the same sample mixture on a Hypersil HyPurity C18 and a Symmetry C18 column under identical mobile phase conditions. Even though both columns are packed with base deactivated C18 stationary phases, the band spacing (selectivity) between peaks is very different on the two columns. Without more information, it is impossible to predict how the performance of different stationary phases will compare.

Hplc Column Comparison Guide

This Comparison Guide to C18 Reversed Phase HPLC Columns provides basic comparison information on commonly used C18 columns to help you more easily identify similarities and differences before investing time and money in chromatographic testing. Hopefully, this information will help you find the right column for your application quicker. Only silica based C18 bonded phases are evaluated in this Guide. Other bonded phases, such as C8, CN, Phenyl and polar embedded phases, are excluded. This Guide does not identify an overall “best” column. The column that works best for one application will not necessarily be the column that will work best for other applications. And, there certainly is not a single column that will work best for all applications.

Hplc Column Comparison Guide

However, this Guide can help you identify columns that are likely to perform well so that at least you can narrow the number of columns for chromatographic testing. You may find that this Guide helps you identify several columns that provide good separations and performance. It is always desirable to have more than one column identified for an application, especially if you are running routine assays. Increasingly, chromatographers are seeking to identify alternate brands of HPLC columns suitable for their assays. Having an alternate column choice for a method reduces the risk of “down time” due to column problems such as a change in selectivity from one manufactured lot to another or slow supplier delivery.

Finding an alternate or back-up column that will provide acceptable selectivity and performance when substituted into a method can be as expensive and time consuming as finding the right column for developing an initial separation. It is our hope that this Guide will make that job easier by identifying columns with similar chromatographic characteristics.

Stationary Phase Specifications | Phases Compared According to Relative Hydrophobicity | Phases Compared According to Relative Polarity | Categorization of Phases According to Hydrophobicity and Polarity | Comparison of Column Efficiency for a Neutral Compound | Comparison of Column Efficiency for Basic Compounds | Phases Grouped According to Silanol Activity | Comparison of Phases According to Metal Activity