high performance liquid chromatography, broad peaks

Factors affecting resolution in chromatography

The resolution of elution is a quantitative measurement that how two peak peaks can be separated into a chromatographic separation. It is defined as the dissimilarity in the RT among two peaks, which is divided by the combined width of the elution peaks. The resolution in chromatography is calculated by the equation below. Rs = 2(t2-t1)/w1+w2 The equation shows that t1 and t2 are the retention time of components first and second simultaneously, while w1 and w2 are bandwidth at the base. The equation of the resolution specifies that the resolution can be affected by three significant parameters. 

Separation factor (Selectivity): the chromatographic system can differentiate chemically amongst sample analytes. Efficiency: The efficiency in chromatography is calculating the dispersion of the sample the component band as it passes through the HPLC column and system.

Capacity factor (k) (Retention factor): The capacity factor is calculating the retention of a sample component on the column. 


Go up