what is hplc

Difference between Absorption and Emission Spectrum

Analytical spectroscopy consists of two major categories of instruments, depending on atomic absorption and atomic emission. Inductively Coupled Plasma (ICP) Spectrometer is an example of an instrument atomic nuclear emission and atomic absorption spectrometer is an example that uses absorption energy, these instrument use technologies depend on the quantum mechanics, where electrons transmit separate transitions from the one energy stage to another stage.

Absorption Spectra:The spectrum acquires by transmitting electromagnetic radiation by a matter or substance called as absorption spectra.The electrons of an element which are in the ground stage can absorb the phenomena, energy to attain a higher energy state. The light frequencies are transmitted through this matter or substance, the dark band shows the absorbed light, form the absorption spectrum of the matter.

Emission Spectra:A spectrum of electromagnetic radiation emitted by a matter or substance called as emission spectra.At the point when heat, light is as energy given to a component or element, the electrons of its molecules or atoms acknowledge the energy and go to superior energy levels. But, these electrons have to emit energy to return to their ground conditions, as the excited state is not stable. In such a case, the emitted light frequency forms the emission spectrum.


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