what is hplc

Definition of Partition column chromatography

Column partition chromatography is not widely used technology like column chromatography, but all techniques and requirements are similar to column chromatography. This chromatography technique is parallel to the adsorption column chromatography, excluding that the stationary phase is liquid. The silica gel or cellulose is used as a solid support to grasp the stationary phase (liquid) like aqueous buffer solutions or water as on the surface like a thin film. In this type of chromatography mobile phase is parallel to the column chromatography; however, in this technique gradient elution is not used, since the equilibrium will be upset.


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