HPLC Basics – All about Basics Principles of Chromatography
HPLC basics information HPLC basics information on liquid chromatography in general and HPLC in particular. 1.0 The HPLC 2.0 Types…
Various advantages of HPTLC over HPLC
Both HPTLC and HPLC are well-recognized for the separation of analytes and for qualitative and quantitative analysis and they have…
Types of HPLC columns
To classify liquid chromatography, there are several ways. If this classification relies upon nature of the separation process and the…
The Principle of Affinity Chromatography
In the affinity chromatography, the proteins are separated as per the dissimilarity in their binding affinity. There is a high…
Selection of buffer in HPLC method development
To make an aqueous mobile phase is the most important stage of RP-HPLC method development for ionic molecules. This contains…
Sample Injection System in HPLC
HPLC sample injector is a technique used to load and inject the sample solutions into the HPLC system. This method is…
Role of pKa in HPLC method development
The physicochemical properties of an analyte play a significant role in HPLC method development. Chromatographic method development requires studying the…
Rheodyne injector in HPLC
Rheodyne injector, septum injector, and stop flow are the types of injectors available for HPLC. The working pressure of high-performance liquid chromatography…
Principle and Procedure of Thin Layer Chromatography
What is Thin Layer Chromatography?Thin-layer chromatography is a technique in which the analytes of a mixture are separated by differential…
Principle and Procedure of Paper chromatography
What is Paper Chromatography?Paper chromatography is one of the kinds of processes that operate on a piece of special paper.…