Various advantages of HPTLC over HPLC

25/05/2023

Both HPTLC and HPLC are well-established for analyte separation and qualitative and quantitative analysis and offer many advantages.

Here are some advantages of HPTLC over HPLC: 

  • Preparing samples is easy.
  • Low maintenance cost.
  • The entire spectrum can be seen.
  • Many analysts can work together on the same system.
  • Very low solvent consumption per sample.
  • The solvent does not require any pretreatment, such as filtration and degassing.
  • Samples do not require cleaning.
  • A cloudy sample has no effect on the analysis.
  • A cloudy sample has no effect on the analysis.
  • When using a freshly prepared mobile phase, there is no interference from previous analyses.
  • Analytical grade solvents are acceptable.
  • No expensive solvents are required.
  • UV absorbing and corrosive mobile phases can also be used.
  • Great flexibility in each step of mobile phase selection, stationary phase selection, and development techniques.
  • More basic pH values ​​can be used for mobile phases that are not suitable for HPLC.
  • Analysis requirements are minimum requirements.
  • The technology is simple, no need for professionals to learn and operate.
  • Pre-coated HPTLC boards are inexpensive.
  • The solvent solution is not particularly important for sample solubility because it is completely removed before developing the chromatogram.
  • Samples and standards can be processed simultaneously under the same conditions, providing better analytical accuracy and precision.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting.Perfil Linkedin

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