Both HPTLC and HPLC are well-established for analyte separation and qualitative and quantitative analysis and offer many advantages.
Here are some advantages of HPTLC over HPLC:
- Preparing samples is easy.
- Low maintenance cost.
- The entire spectrum can be seen.
- Many analysts can work together on the same system.
- Very low solvent consumption per sample.
- The solvent does not require any pretreatment, such as filtration and degassing.
- Samples do not require cleaning.
- A cloudy sample has no effect on the analysis.
- A cloudy sample has no effect on the analysis.
- When using a freshly prepared mobile phase, there is no interference from previous analyses.
- Analytical grade solvents are acceptable.
- No expensive solvents are required.
- UV absorbing and corrosive mobile phases can also be used.
- Great flexibility in each step of mobile phase selection, stationary phase selection, and development techniques.
- More basic pH values can be used for mobile phases that are not suitable for HPLC.
- Analysis requirements are minimum requirements.
- The technology is simple, no need for professionals to learn and operate.
- Pre-coated HPTLC boards are inexpensive.
- The solvent solution is not particularly important for sample solubility because it is completely removed before developing the chromatogram.
- Samples and standards can be processed simultaneously under the same conditions, providing better analytical accuracy and precision.