Both HPTLC and HPLC are well-recognized for the separation of analytes and for qualitative and quantitative analysis and they have many advantages.
Here are some of the advantages of HPTLC on HPLC as follows:
- Preparation of the sample is simple.
- Maintenance costs are low.
- The whole spectrum can be seen.
- Many analysts can work together on the same system.
- Consumption of solvents per sample is very low.
- Solvents do not need any prior treatment, such as filtration and degassing.
- There is no need to clean the sample.
- There is no effect of the turbid sample on analysis.
- Using the freshly prepared mobile phase, there is no chance of interference from the previous analysis.
- Analytical grade solvents are suitable. There is no need for expensive solvents.
- UV absorption and Corrosive mobile phase can also be used.
- High flexibility in the different steps, such as the selection of mobile phase, stationary phase, and developing techniques.
- More basic pH can be used in a mobile phase, which is not suitable in HPLC.
- An analytical requirement is least.
- Technically simple, no skilled person is required to learn and operate.
- Precoated HPTLC plates are available in low cost.
- The solution of the solvent is not extremely significant for sample solubility because it is completely removed prior to developing chromatogram.
- Sampling and standard can be processed simultaneously under the same condition provide better analytical accuracy and precision.