LABORATORY PIPETTE: What is it, Types, Use, Function, Care
Although the Moderna laboratory pipette has only existed since the late 1950s, pipettes as scientific tools have existed in some form since the late 1800s. First created by the French biologist Louis Pasteur, who invented the pasteurization process, pasteur pipettes (transfer pipettes) can be used to aspirate and dispense liquids without fear of contamination.
Unfortunately, Pasteur's tools did not catch on quickly because any scientist who wanted to use pipettes would have to create his own personal set of glass. Many continued to use the tried and true - and incredibly dangerous - method of mouth pipetting, where scientists will transfer liquids using straws and their own mouths, even if that liquid was toxic or radioactive.
It was not until the late 1950s that the former German soldier Henrich Schnitger, who hated the practice of pipetting with his mouth, the Moderna mass-produced pipette was developed. These, fortunately, would quickly catch on.
🔍 What is a laboratory pipette and what is it for?
A pipette is a laboratory utensil usually used in chemistry, biology and medicine to transfer a liquid volume. Pipettes come in various designs for various purposes with different levels of accuracy and precision, from one-piece glass pipettes to more complex adjustable electronic pipettes. Many types of pipettes work by creating a partial vacuum over the liquid holding chamber and selectively releasing this vacuum to extract and dispense liquid. The accuracy of the measurement varies greatly depending on the style
⭐ What are the main uses of the pipette?
The serological pipette is a laboratory instrument that transfers liquids measured in volume per ml. Most have graduations on the side to measure the liquid being dispensed or aspirated. An important step when growing or plating cells is uniform distribution throughout the solution.
The use of serological pipettes is an effective and gentle means for mixing cell suspensions. They can also be used to mix reagents and chemical solutions. After treatment or isolation of experimental cell cultures, serological pipettes are useful for transferring colonies of cells for further empirical analysis or expansion.
In addition, they are used to carefully reactivate reagents to create density gradients, such as a phycoline gradient that is used to purify plasma cells.
Serological pipettes are available in many options and sizes. There are four varieties of disposable serological pipettes. Calibrated pipettes deliver volumes ranging from 0.1 ml to 25 ml. The most common sizes are 5, 10 and 25 ml.
They are used for liquid transfers that are free of pathogenic microorganisms. For microbiology and tissue culture experiments, Next Day Science has pre-sterilized transfer pipettes that can be heat-sealed to store airtight samples.
Common uses of laboratory pipettes are:
* Blood bank
They are excellent for applications involving tissue cultures. Low affinity surfaces reduce the loss of samples, proteins or cells. These pipettes come in ten designs. It is very likely that one of them will meet the requirements for a number of drops per ml, pipette capacity, bulb extraction or length.
Plastic pipettes can be used for biosafety level in laboratory experiments (BSL-1). Plastic pipettes are the only type suitable for BSL-2 organisms where Bunsen burners cannot be used. They are also recommended for applications involving fluid agar transfer.
⌛ Types of pipettes
Pipettes are used in scientific laboratories. Its essential intention is to suck a liquid that the consumer wants from the laboratory and then keep the liquid so that it can be transported to another container or container. Some pipettes are not very accurate and are intended more for transfer than for measuring liquids, while others are very accurate and measure the volume of liquid.
Volumetric pipettes are used to transfer a specific volume of a given liquid. It usually has a capacity of between 1 and 100 ml. They can have the shape of a rolling pin, with two thinner ends and a thicker protrusion in the middle. These are used when the accuracy in measuring the transferred liquid is important for registration.
Measuring pipettes are straight tubes with a tapering end. They have clearly marked control marks along the side of the tube, so multiple amounts of liquid can be measured with a single pipette. These types of pipettes can usually measure a volume between 0.1 mL and 25 mL. While they can measure multiple amounts of liquid at once, imperfections in the inner diameter of their tube mean that they are not as accurate in their measurements as volumetric pipettes.
Mohr and serological pipettes
Measuring pipettes are subdivided into Mohr pipettes and serological pipettes. The difference between these two types is that the Mohr pipette control marks, or gradations, always end before the pipette tip, while serological pipettes have gradations that continue towards the tips. In addition, some serological pipettes are also blowing pipettes.
These pipettes are similar to a straw, where the client holds his thumb over the top area to establish the vacuum and fix the liquid in the pipette. Removable pipettes allow you to fly into this open end to get the last pieces of liquid left in the pipette into its receiving container, for greater accuracy.
Melting pipettes are clearly marked with a frosted band or two thin rings around the neck. Do not confuse a manufacturer's color code with the markings of a blowing pipette. While you can use the blow pipettes as intended, it is dangerous to do so with a pipette that is not clearly marked as a burst.
Laboratory pipette use
Most of our work is done with sterile pipettes, and if so, the operations are performed aseptically. When working with non-sterile pipettes, it is a good idea to work aseptically anyway as a matter of course to practice the technique.
• Without opening the sterile sleeve, look through the wrapper and verify that the pipette is calibrated as a "blow" pipette.
• Also make sure that the tip is not cracked or chipped and check that the wrap has not suffered any damage.
• Open the wrapper and remove the pipette aseptically and insert the upper and wide end into a pipette helper.
* Fill the pipette a little above the desired capacity line and then slowly lower the meniscus to that capacity line.
* Remove the pipette from the container, allowing the outside of the pipette to gently touch the inner edge of the container to remove any adhering liquid.
* Do not touch the tip of the pipette to avoid introducing an air bubble.
* Aseptically move the pipette to the receiving container and deliver the contents.
• If you are pipetting a volume between two measuring lines, you will not have to "blow". However, if you are supplying the entire contents of the pipette, you will have to "blow out" the remaining liquid at the tip with a firm puff of air from the pipette aid.
* Remove the pipette aseptically and dispose of it in a suitable waste container.
🍀 Laboratory pipette function
They are created to transport a certain amount of liquid with great accuracy. These pipettes can only be used to supply the volume of liquid for which it is calibrated. Volumetric pipettes have narrow tips and a bulb-like expansion in the middle. The unique calibration mark for these pipettes is located in the section of the tube above the center expansion.
🌻 Laboratory pipette care
Pipettes require cleaning after each use, to ensure that they are accurate and to prevent contamination of any previous contents. To clean one, insert distilled water into the pipette and tilted, so that the water makes contact with the inner surface of the pipette. Repeat this process twice, then rinse the entire pipette with distilled water to finish cleaning it.
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