Paper chromatography is a chromatographic method of separation in which the flow of solvent separates the substance by capillary action on the specially designed paper (cellulose filter paper) that acts as a stationary phase. The quantity of each analyte of the sample mixture can be determined using retention factors (Rf). Retention factors are helpful in the comparison between the chromatograms.Here are some factors that affect the Rf value of paper chromatography.
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