Difference Between Fluorescence and Absorbance

The main difference between fluorescence and absorbance is that absorption is the process that eats up a photon and puts a molecule or atom into an excited state. Fluorescence is the process that first devours a photon and places a molecule or atom in an excited state, then emits low energy photons that transport the molecule or atom to the ground state.

The amount of light absorbed by a substance is measured in absorption spectrometer while fluorescence spectrometry measures the intensity or amount of light emitted by a molecule.

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Jose Hullgren (Laboratory Analist)

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