Fluorometry is an analytical device for the determination and measurement of fluorescence in compounds using ultraviolet light in compounds. It is an analytical method to detect and measure fluorescence in molecules using light from fluorescence in a molecule. The fluorometry technique is mainly applied to low concentration ranges and is therefore a sensitive analytical method compared to spectrophotometric determination. Typically, fluorometric methods have sensitivities of two to four orders of magnitude and higher compared to spectrophotometric methods.
The advantages of fluorimetry are as follows.
- The main advantage of fluorimetry is its high sensitivity compared to other absorption methods.
- Fluorometry has high specificity due to the unique optical properties of compounds.
- This will measure the fluorescence intensity and concentration of the sample.
- More precise on the characteristics of the excitation and emission wavelengths.
- It is more accurate than the absorption process because the emission and excitation wavelengths are characteristic.
- Fluorimetry has a wide range of concentrations.
- It is an easy to use and inexpensive method.
The disadvantages of fluorimetry are as follows.
- The main disadvantage of the fluorescence method is that it only analyzes fluorescent molecules, not all of them.
- A rapid scan is not possible to obtain the excitation and emission spectra of the analyte.
- The reference and the sample solution cannot be analyzed at the same time.
- It is not useful for identification.
- Solutions of dilute sample compounds are less stable.