An HPLC instrumentation can be a set of individual modules or elements, but it can bedesigned as a single apparatus as well. The module concept is more flexible in the caseof the failure of a single component; moreover, the individual parts need not be fromthe same manufacturer. If you do not like to do minor repairs by yourself you willprefer a compact instrument. This, however, does not need less bench space than amodular set.
An HPLC instrument has at least the elements in figure 1.3 : solvent reservoir, transfer line with frit, high-pressure pump, sample injection device,column, detector, and data acquisition, usually together with data evaluation.
Although the column is the most important part, it is usually the smallest one. For temperature-controlled separations it is enclosed in a thermostat. It is quite common to work with more than one solvent, thus a mixer and controller are needed. If the data acquisition is done by a computer it can also be used for the control of the whole system. Hplc instrumentation is typically made up of nine basic components: mobile phase/solvent reservoir, solvent delivery system, sample introduction device, column, post-column apparatus, detector, data collection and output system, post-detector eluent processing, and connective tubing and fittings
This can be done by using following techniques:
The introduction of a sample in HPLC is a technique to inject the samples without interrupting the pressure and flow rate of the HPLC system. HPLC’s work pressure is sufficiently high so that we cannot introduce a sample in the mobile phase by injecting the syringe, that’s why we require an injector that gives reproducible results and without disturbing the system pressure and flow rate. Nowadays, modern injectors are autosamplers, that permit programmed injection of various volumes of sample, which takes from the vials in autosampler trays. Rheodyne injector, septum injector, and stop flow injector are the types of the HPLC injector.
Here are some types of HPLC detectors mentioned.