Advantages and disadvantages of fluorescence spectroscopy
The advantages of fluorescence spectroscopy are as follows.
- Its high sensitivity is the main advantage of fluorimetry.
- Due to the unique optical properties of the component, it has high specificity.
- You can determine the fluorescence intensity, decay time, and component concentration.
- May be insensitive to light scatter.
- Emitted light is read at the correct angle to the exciting light, reducing background signal
- These types of methods have a wide range of linearity.
The disadvantages of fluorescence spectroscopy are as follows.
- The main disadvantage of fluorescence spectroscopy is that not all molecules fluoresce.
- It has limitations related to loss of recognition ability and photostability.
- Sensitive to interference from changes in sample pH and oxygen levels.
- It is sensitive to sample autofluorescence.
- Problem related to potential toxicity, due to foreign bodies in biological media.
- The short life of fluorophores is another disadvantage of fluorometry.
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