Advantages and disadvantages of fluorescence spectroscopy

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  1. Advantages and disadvantages of fluorescence spectroscopy
    1. The advantages of fluorescence spectroscopy are as follows.
    2. The disadvantages of fluorescence spectroscopy are as follows.

Advantages and disadvantages of fluorescence spectroscopy


The advantages of fluorescence spectroscopy are as follows.

  • Its high sensitivity is the main advantage of fluorimetry.
  • Due to the unique optical properties of the component, it has high specificity.
  • You can determine the fluorescence intensity, decay time, and component concentration.
  • May be insensitive to light scatter.
  • Emitted light is read at the correct angle to the exciting light, reducing background signal
  • These types of methods have a wide range of linearity.

The disadvantages of fluorescence spectroscopy are as follows.

  • The main disadvantage of fluorescence spectroscopy is that not all molecules fluoresce.
  • It has limitations related to loss of recognition ability and photostability.
  • Sensitive to interference from changes in sample pH and oxygen levels.
  • It is sensitive to sample autofluorescence.
  • Problem related to potential toxicity, due to foreign bodies in biological media.
  • The short life of fluorophores is another disadvantage of fluorometry.

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Jose Hullgren (Laboratory Analist)

Hello to all readers, my name is Jose Hullgren, it is a pleasure to present you this website of my authorship, I am currently working as a laboratory analyst and for the last 10 years I have been working in the pharmaceutical industry. The main idea of this page is to provide relevant information in the field of the pharmaceutical industry above all. We also cover different areas of chemistry and sciences in general that we find interesting. Perfil Linkedin

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