Advantages and disadvantages of fluorescence spectroscopy

The advantages of fluorescence spectroscopy are as follows.

• Its high sensitivity is the main advantage of fluorimetry.
• Due to the unique optical properties of the component, it has high specificity.
• You can determine the fluorescence intensity, decay time, and component concentration.
• May be insensitive to light scatter.
• Emitted light is read at the correct angle to the exciting light, reducing background signal
• These types of methods have a wide range of linearity.

The disadvantages of fluorescence spectroscopy are as follows.

• The main disadvantage of fluorescence spectroscopy is that not all molecules fluoresce.
• It has limitations related to loss of recognition ability and photostability.
• Sensitive to interference from changes in sample pH and oxygen levels.
• It is sensitive to sample autofluorescence.
• Problem related to potential toxicity, due to foreign bodies in biological media.
• The short life of fluorophores is another disadvantage of fluorometry.