Principle and Procedure of HPLC
What is High-Performance Liquid Chromatography? HPLC is an analytical technique in chemistry for the separation, identification, and quantification of the…
Band Broadening – four causes that we can solve
Band broadening: there are many reasons for and it is important that these are understood and the phenomenon kept to…
Principle and Procedure of Column Chromatography
What is Column Chromatography?Column chromatography is one of the most effective methods for separating and purifying solid and liquid samples,…
Principle and Procedure of Affinity Chromatography
What is Affinity Chromatography? Affinity chromatography is a form of liquid chromatography used to isolate and purify analytes in a…
Principle and Procedure of Adsorption Chromatography
What is Adsorption Chromatography? Adsorption Chromatography Principle Adsorption chromatography works on the principle that certain solid material, called adsorbent can…
Principle and Application of Partition Chromatography
What is Partition Chromatography? Partition chromatography is a type of chromatography in which the analytes of a sample mixture distribute…
Pharmaceutical Applications of Column Chromatography
Column chromatography is a separation technique that is used to purify compounds on the basis of their hydrophobicity or polarity.…
Mobile phase selection in LC-MS
The use a volatile mobile phase is a good starting point. As well as essential mobile phases such as methanol,…
Mobile Phase of HPLC
The retention time of analytes in HPLC is managed by the polarity of the mobile phase. The mobile phase of HPLC…
Method development by HPLC
Method development is the procedure of confirming that an analytical method is suitable for applying in the research laboratory to…