¿What are the causes of broad peaks in HPLC?
The presence of unusually broad peaks is sometimes an occurring problem in liquid chromatography separation. This problem is regularly seen…
¿What are the Applications of Thin Layer Chromatography?
Thin-layer chromatography (TLC) is a broadly used method in the study of chemistry. It is used to separate and evaluate…
¿What are the Applications of Paper Chromatography?
Paper chromatography is an analytical technique used to separate the components of complex mixtures. It is one of the types…
¿What are the Applications of Chromatography?
Chromatography is the technique for separating analytes or a mixture depending on the relative affinity of each analyte distributed between…
¿What are the common buffers used in HPLC?
High-performance liquid chromatography is used to separate molecules; it is depending on their affinity towards the stationary phase. Symmetric peak…
¿What are the advantages of TLC over HPLC chromatography?
Thin-layer chromatography (TLC) is used for the separation and quantification of analytes in the form of a mixture of components…
¿What are the Advantages of HPLC over GC?
High-performance liquid chromatography and Gas chromatography both are the separation techniques for the components in analytical chemistry and they are…
¿How to select a pH of mobile phase in HPLC?
When an ionizable compound in a sample, the pH of the mobile phase or buffer is the most significant variable…
¿How to select a column for HPLC method development?
In high-performance liquid chromatography, to separate the components is our object and this occurs in the column (Stationary Phase), hence the column…
¿How to reduce peak broadening in HPLC?
Peak broadening is an occurrence in high-performance liquid chromatography separation that decreases separation efficiency causes leading to poor resolution of analytes. The…